Support in study design, execute, and analyze experiments for upstream polysaccharide for bacterial fermentation (BSL2) and vaccine production processes. - Execute experiments to develop the upstream process parameters (e.g., media, feed, pH, temperature, dissolved oxygen, agitation, etc.) and to enhance product yield, quality, and consistency. - Perform microbiology related work including plating, colony selection, Gram staining and cell enumeration on microscope. - Perform fermentation vessel assembly and script writing for process automation and execute fermentation experiment in shake-flask and lab scale bioreactors. - Prepare media and buffer as needed for experiments. - Operate and maintain instrumentation for bacterial fermentation and primary recovery (centrifugation, filtration, etc.) of bacterial polysaccharides utilizing aseptic techniques. - Support analytical method execution including optical density, residual metabolites and polysaccharide concentration and other relevant analytical techniques. - Assist in executing process development studies. - Keep accurate and current records of research and/or project related activities in laboratory notebooks and Electronic Lab Notebook. - Prepare and review protocol, technical reports, SOPs, and batch records. - Present and communicate scientific data and results to internal stakeholders. - Be an outstanding teammate and collaborate effectively with cross functional team members. - and collaborate effectively with downstream, analytical, and manufacturing teams within Polysaccharide Development and Manufacturing team to ensure seamless technology transfer and process integration. ### Requirements:
MSc in Chemical/Biochemical Engineering, Biotechnology, or similar degree with a minimum of 3 years of relevant industry experience, or BSc with a minimum of 5 years of relevant industry experience. - Experience in handling microorganisms with proper aseptic techniques to perform upstream processing related work in flasks/bioreactors. - Hands-on experience and theoretical understanding of key processing operations such as microbial culture, fermentation process, microbial media, and process optimization and relevant analytical technologies; experience with depth filtration, continuous centrifugation is a plus. - Develop and optimize scripts for instrumentation, including Ambr® systems, to enhance efficiency and automate processes, leveraging programming languages such as Python or R is a plus. - Experience with Design of Experiments (DoE) principles and DoE software. - Process characterization experience is a plus. - Cell banking expertise, colony selection and genetic analysis of recombinant strains is a plus. - Proficiency with computer programs such as Microsoft Excel, Word, PowerPoint, Visio and JMP. - Working knowledge of GMP requirements. - Proven ability to succeed in a cross-functional team environment, effectively managing multiple projects and contributing to team outcomes. - Strong interpersonal skills, with excellent written and verbal communication abilities. Reports to: Senior Scientist, Upstream Polysaccharide Process Development
